Journal: PLOS One
Article Title: SHP2 improved Late-onset fetal growth restriction via modulating ROS/BRD4/PI3K/YAP/PIGF signaling induced angiogenesis
doi: 10.1371/journal.pone.0342649
Figure Lengend Snippet: A: Western blot analysis of NOX4, nuclear BRD4, p-SHP2, p-PI3K, nuclear YAP, Nrf2, and PIGF protein expressions in EPCs in the following six groups: NC group, Model group, Model + JQ-1 group, Model + JQ-1 + PHPS1 group, Model + JQ-1 + PHPS1 + 740Y-P group, and Model + JQ-1 + PHPS1 + 740Y-P + Verteporfin group, GAPDH as the control protein; B: Statistical analysis of relative protein expression levels. N = 3; Data are expressed as mean ± standard deviation; Different lowercase letters on the same column indicate significant differences between groups at P < 0.05, while different uppercase letters indicate significant differences at P < 0.01.
Article Snippet: Rabbit anti-NOX4 antibody, nuclear BRD4 antibody, phospho §-SHP2 antibody, p-PI3K antibody, nuclear YAP antibody, Nrf2 antibody, PIGF antibody, VEGF antibody, HIF1α antibody, OCT4 antibody, SOX2 antibody, and C-Myc antibody were purchased from Wuhan Sanying Biotechnology Co., Ltd. Rabbit secondary antibody, RIPA lysis buffer, and ECL luminescence solution were purchased from Beijing Lanjie Technology Co., Ltd.
Techniques: Western Blot, Control, Expressing, Standard Deviation